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rabbit polyclonal anti-homer2 antibody  (Synaptic Systems)


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    Synaptic Systems rabbit polyclonal anti-homer2 antibody
    (A) Workflow of the <t>Anti-Homer2</t> co-IP experiment. The different WT and KO fractions are colored in blue and red, respectively. (B) 2-D western blot images of a representative input fraction (top) and a representative co-IP sample (bottom) blotted for Homer2a and Homer2b proteins. (C) 1-D western blot image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples and blotted for Homer2 proteins. (D) 1-D silver stained gel image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples. Lanes in (A), (C), and (D) correspond to: (L), ladder; (1), Homogenate; (2), Pellet; (3), Supernatant (input fraction); (4), Pre-cleared supernatant; (5), Post-co-IP supernatant; and (6) co-IP sample. MW = molecular weight
    Rabbit Polyclonal Anti Homer2 Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-homer2+antibody/pmc05722633-68-9-17?v=Synaptic+Systems
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-homer2 antibody - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "A mass spectrometry-based proteomic analysis of Homer2-interacting proteins in the mouse brain"

    Article Title: A mass spectrometry-based proteomic analysis of Homer2-interacting proteins in the mouse brain

    Journal: Journal of proteomics

    doi: 10.1016/j.jprot.2017.07.008

    (A) Workflow of the Anti-Homer2 co-IP experiment. The different WT and KO fractions are colored in blue and red, respectively. (B) 2-D western blot images of a representative input fraction (top) and a representative co-IP sample (bottom) blotted for Homer2a and Homer2b proteins. (C) 1-D western blot image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples and blotted for Homer2 proteins. (D) 1-D silver stained gel image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples. Lanes in (A), (C), and (D) correspond to: (L), ladder; (1), Homogenate; (2), Pellet; (3), Supernatant (input fraction); (4), Pre-cleared supernatant; (5), Post-co-IP supernatant; and (6) co-IP sample. MW = molecular weight
    Figure Legend Snippet: (A) Workflow of the Anti-Homer2 co-IP experiment. The different WT and KO fractions are colored in blue and red, respectively. (B) 2-D western blot images of a representative input fraction (top) and a representative co-IP sample (bottom) blotted for Homer2a and Homer2b proteins. (C) 1-D western blot image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples and blotted for Homer2 proteins. (D) 1-D silver stained gel image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples. Lanes in (A), (C), and (D) correspond to: (L), ladder; (1), Homogenate; (2), Pellet; (3), Supernatant (input fraction); (4), Pre-cleared supernatant; (5), Post-co-IP supernatant; and (6) co-IP sample. MW = molecular weight

    Techniques Used: Co-Immunoprecipitation Assay, Western Blot, Staining, Molecular Weight

    (A) Of the 31 previously reported Homer2-interacting proteins, 11 were detected by MS1 full-scan filtering in the mouse whole-brain fractions used to co-IP Homer2 (yellow), 6 were detected in the co-IP samples (blue), and 14 proteins were not detected in either dataset (gray). (B) A total of 22 proteins were significantly enriched at a 10% FDR in WT co-IP samples. In addition to the four previously reported Homer2-interacting proteins (green), 18 novel proteins co-IPed with Homer2 (orange). FDR = false discovery rate.
    Figure Legend Snippet: (A) Of the 31 previously reported Homer2-interacting proteins, 11 were detected by MS1 full-scan filtering in the mouse whole-brain fractions used to co-IP Homer2 (yellow), 6 were detected in the co-IP samples (blue), and 14 proteins were not detected in either dataset (gray). (B) A total of 22 proteins were significantly enriched at a 10% FDR in WT co-IP samples. In addition to the four previously reported Homer2-interacting proteins (green), 18 novel proteins co-IPed with Homer2 (orange). FDR = false discovery rate.

    Techniques Used: Co-Immunoprecipitation Assay

    (A) Detection of previously reported Homer2-interacting proteins in the input fractions and co-IP samples. (B) Enrichment of previously reported and novel Homer2-interacting proteins in co-IP samples. Previously reported Homer2-interacting proteins that were detected in the input fractions and co-IP samples are colored yellow and blue, respectively. Previously reported and novel Homer2-interacting proteins that were significantly enriched in WT samples are colored green and orange, respectively. Table headers correspond to: (UniProt ID), mouse UniProt protein identifier; (Indistinguishable Group), proteins that contain peptides expressed in more than one gene product; (Gene) common gene name; (Protein Description) protein name; (Common Name) short protein name or alternative protein name; (FC Log2) Log2 transformed fold change; (FDR) false discovery rate (q ≤ 0.10) ; [SRM Verified (+2 peptides)], whether or not the protein was verified by SRM mass spectrometry using +2 charged peptides in a second co-IP experiment. N/A = not applicable.
    Figure Legend Snippet: (A) Detection of previously reported Homer2-interacting proteins in the input fractions and co-IP samples. (B) Enrichment of previously reported and novel Homer2-interacting proteins in co-IP samples. Previously reported Homer2-interacting proteins that were detected in the input fractions and co-IP samples are colored yellow and blue, respectively. Previously reported and novel Homer2-interacting proteins that were significantly enriched in WT samples are colored green and orange, respectively. Table headers correspond to: (UniProt ID), mouse UniProt protein identifier; (Indistinguishable Group), proteins that contain peptides expressed in more than one gene product; (Gene) common gene name; (Protein Description) protein name; (Common Name) short protein name or alternative protein name; (FC Log2) Log2 transformed fold change; (FDR) false discovery rate (q ≤ 0.10) ; [SRM Verified (+2 peptides)], whether or not the protein was verified by SRM mass spectrometry using +2 charged peptides in a second co-IP experiment. N/A = not applicable.

    Techniques Used: Co-Immunoprecipitation Assay, Transformation Assay, Mass Spectrometry

    Cartoon depiction of a neuronal post-synapse. Previously reported Homer2-interacting proteins that were significantly enriched in WT samples are colored green and novel proteins are colored orange. The majority of Homer2-interacting proteins are NMDAR subunits or part of the NMDAR signaling pathway (gray area). Homer2 is not included as an interacting protein because our co-IP experiments cannot differentiate Homer2 proteins directly bound by the antibody from Homer2 homomultimers. Literature references for the involvement of these proteins in the NMDAR signaling pathway are included in Supplemental Table 3.
    Figure Legend Snippet: Cartoon depiction of a neuronal post-synapse. Previously reported Homer2-interacting proteins that were significantly enriched in WT samples are colored green and novel proteins are colored orange. The majority of Homer2-interacting proteins are NMDAR subunits or part of the NMDAR signaling pathway (gray area). Homer2 is not included as an interacting protein because our co-IP experiments cannot differentiate Homer2 proteins directly bound by the antibody from Homer2 homomultimers. Literature references for the involvement of these proteins in the NMDAR signaling pathway are included in Supplemental Table 3.

    Techniques Used: Co-Immunoprecipitation Assay



    Similar Products

    rabbit polyclonal anti-homer2 antibody  (Synaptic Systems)
    90
    Synaptic Systems rabbit polyclonal anti-homer2 antibody
    (A) Workflow of the <t>Anti-Homer2</t> co-IP experiment. The different WT and KO fractions are colored in blue and red, respectively. (B) 2-D western blot images of a representative input fraction (top) and a representative co-IP sample (bottom) blotted for Homer2a and Homer2b proteins. (C) 1-D western blot image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples and blotted for Homer2 proteins. (D) 1-D silver stained gel image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples. Lanes in (A), (C), and (D) correspond to: (L), ladder; (1), Homogenate; (2), Pellet; (3), Supernatant (input fraction); (4), Pre-cleared supernatant; (5), Post-co-IP supernatant; and (6) co-IP sample. MW = molecular weight
    Rabbit Polyclonal Anti Homer2 Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-homer2+antibody/pmc05722633-68-9-17?v=Synaptic+Systems
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-homer2 antibody - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Workflow of the Anti-Homer2 co-IP experiment. The different WT and KO fractions are colored in blue and red, respectively. (B) 2-D western blot images of a representative input fraction (top) and a representative co-IP sample (bottom) blotted for Homer2a and Homer2b proteins. (C) 1-D western blot image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples and blotted for Homer2 proteins. (D) 1-D silver stained gel image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples. Lanes in (A), (C), and (D) correspond to: (L), ladder; (1), Homogenate; (2), Pellet; (3), Supernatant (input fraction); (4), Pre-cleared supernatant; (5), Post-co-IP supernatant; and (6) co-IP sample. MW = molecular weight

    Journal: Journal of proteomics

    Article Title: A mass spectrometry-based proteomic analysis of Homer2-interacting proteins in the mouse brain

    doi: 10.1016/j.jprot.2017.07.008

    Figure Lengend Snippet: (A) Workflow of the Anti-Homer2 co-IP experiment. The different WT and KO fractions are colored in blue and red, respectively. (B) 2-D western blot images of a representative input fraction (top) and a representative co-IP sample (bottom) blotted for Homer2a and Homer2b proteins. (C) 1-D western blot image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples and blotted for Homer2 proteins. (D) 1-D silver stained gel image of fractions collected from a representative co-IP experiment performed using WT (left) and KO (right) mouse whole-brain samples. Lanes in (A), (C), and (D) correspond to: (L), ladder; (1), Homogenate; (2), Pellet; (3), Supernatant (input fraction); (4), Pre-cleared supernatant; (5), Post-co-IP supernatant; and (6) co-IP sample. MW = molecular weight

    Article Snippet: The samples were co-IPed with 10 μg of a rabbit polyclonal anti-Homer2 antibody (catalog # 160 203, Synaptic Systems, Göttingen, Germany) and 100 μl Protein A-Sepharose 4B Conjugate beads by nutating at 4 °C for 12 h and 6 h, respectively.

    Techniques: Co-Immunoprecipitation Assay, Western Blot, Staining, Molecular Weight

    (A) Of the 31 previously reported Homer2-interacting proteins, 11 were detected by MS1 full-scan filtering in the mouse whole-brain fractions used to co-IP Homer2 (yellow), 6 were detected in the co-IP samples (blue), and 14 proteins were not detected in either dataset (gray). (B) A total of 22 proteins were significantly enriched at a 10% FDR in WT co-IP samples. In addition to the four previously reported Homer2-interacting proteins (green), 18 novel proteins co-IPed with Homer2 (orange). FDR = false discovery rate.

    Journal: Journal of proteomics

    Article Title: A mass spectrometry-based proteomic analysis of Homer2-interacting proteins in the mouse brain

    doi: 10.1016/j.jprot.2017.07.008

    Figure Lengend Snippet: (A) Of the 31 previously reported Homer2-interacting proteins, 11 were detected by MS1 full-scan filtering in the mouse whole-brain fractions used to co-IP Homer2 (yellow), 6 were detected in the co-IP samples (blue), and 14 proteins were not detected in either dataset (gray). (B) A total of 22 proteins were significantly enriched at a 10% FDR in WT co-IP samples. In addition to the four previously reported Homer2-interacting proteins (green), 18 novel proteins co-IPed with Homer2 (orange). FDR = false discovery rate.

    Article Snippet: The samples were co-IPed with 10 μg of a rabbit polyclonal anti-Homer2 antibody (catalog # 160 203, Synaptic Systems, Göttingen, Germany) and 100 μl Protein A-Sepharose 4B Conjugate beads by nutating at 4 °C for 12 h and 6 h, respectively.

    Techniques: Co-Immunoprecipitation Assay

    (A) Detection of previously reported Homer2-interacting proteins in the input fractions and co-IP samples. (B) Enrichment of previously reported and novel Homer2-interacting proteins in co-IP samples. Previously reported Homer2-interacting proteins that were detected in the input fractions and co-IP samples are colored yellow and blue, respectively. Previously reported and novel Homer2-interacting proteins that were significantly enriched in WT samples are colored green and orange, respectively. Table headers correspond to: (UniProt ID), mouse UniProt protein identifier; (Indistinguishable Group), proteins that contain peptides expressed in more than one gene product; (Gene) common gene name; (Protein Description) protein name; (Common Name) short protein name or alternative protein name; (FC Log2) Log2 transformed fold change; (FDR) false discovery rate (q ≤ 0.10) ; [SRM Verified (+2 peptides)], whether or not the protein was verified by SRM mass spectrometry using +2 charged peptides in a second co-IP experiment. N/A = not applicable.

    Journal: Journal of proteomics

    Article Title: A mass spectrometry-based proteomic analysis of Homer2-interacting proteins in the mouse brain

    doi: 10.1016/j.jprot.2017.07.008

    Figure Lengend Snippet: (A) Detection of previously reported Homer2-interacting proteins in the input fractions and co-IP samples. (B) Enrichment of previously reported and novel Homer2-interacting proteins in co-IP samples. Previously reported Homer2-interacting proteins that were detected in the input fractions and co-IP samples are colored yellow and blue, respectively. Previously reported and novel Homer2-interacting proteins that were significantly enriched in WT samples are colored green and orange, respectively. Table headers correspond to: (UniProt ID), mouse UniProt protein identifier; (Indistinguishable Group), proteins that contain peptides expressed in more than one gene product; (Gene) common gene name; (Protein Description) protein name; (Common Name) short protein name or alternative protein name; (FC Log2) Log2 transformed fold change; (FDR) false discovery rate (q ≤ 0.10) ; [SRM Verified (+2 peptides)], whether or not the protein was verified by SRM mass spectrometry using +2 charged peptides in a second co-IP experiment. N/A = not applicable.

    Article Snippet: The samples were co-IPed with 10 μg of a rabbit polyclonal anti-Homer2 antibody (catalog # 160 203, Synaptic Systems, Göttingen, Germany) and 100 μl Protein A-Sepharose 4B Conjugate beads by nutating at 4 °C for 12 h and 6 h, respectively.

    Techniques: Co-Immunoprecipitation Assay, Transformation Assay, Mass Spectrometry

    Cartoon depiction of a neuronal post-synapse. Previously reported Homer2-interacting proteins that were significantly enriched in WT samples are colored green and novel proteins are colored orange. The majority of Homer2-interacting proteins are NMDAR subunits or part of the NMDAR signaling pathway (gray area). Homer2 is not included as an interacting protein because our co-IP experiments cannot differentiate Homer2 proteins directly bound by the antibody from Homer2 homomultimers. Literature references for the involvement of these proteins in the NMDAR signaling pathway are included in Supplemental Table 3.

    Journal: Journal of proteomics

    Article Title: A mass spectrometry-based proteomic analysis of Homer2-interacting proteins in the mouse brain

    doi: 10.1016/j.jprot.2017.07.008

    Figure Lengend Snippet: Cartoon depiction of a neuronal post-synapse. Previously reported Homer2-interacting proteins that were significantly enriched in WT samples are colored green and novel proteins are colored orange. The majority of Homer2-interacting proteins are NMDAR subunits or part of the NMDAR signaling pathway (gray area). Homer2 is not included as an interacting protein because our co-IP experiments cannot differentiate Homer2 proteins directly bound by the antibody from Homer2 homomultimers. Literature references for the involvement of these proteins in the NMDAR signaling pathway are included in Supplemental Table 3.

    Article Snippet: The samples were co-IPed with 10 μg of a rabbit polyclonal anti-Homer2 antibody (catalog # 160 203, Synaptic Systems, Göttingen, Germany) and 100 μl Protein A-Sepharose 4B Conjugate beads by nutating at 4 °C for 12 h and 6 h, respectively.

    Techniques: Co-Immunoprecipitation Assay